Premium
Patterns of transgene inheritance in rainbow trout ( Oncorhynchus mykiss )
Author(s) -
Penman D. J.,
Iyengar A.,
Beeching A. J.,
Rahman A.,
Sulaiman Z.,
Maclean N.
Publication year - 1991
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080300306
Subject(s) - rainbow trout , biology , inheritance (genetic algorithm) , transgene , genetics , trout , zoology , fishery , fish <actinopterygii> , evolutionary biology , gene
There have been very few studies of the inheritance of introduced genes (transgenes) in fish. We have followed the inheritance of the mammalian fusion gene MTrGH from founder generation transgenics (originating from eggs microinjected with the MTrGH DNA) to offspring in crosses with control fish. Initial screening of the founder generation transgenics was by analysing DNA from blood samples. Only three out of six fish which carried the novel gene in blood DNA transmitted it to their offspring, despite the presence of the gene in DNA extracted from the sperm of all four male fish in this group. The frequency of transgenics in the progeny groups from the three fish which transmitted the gene varied widely: in one of these groups more than one type of MTrGH restriction pattern was found. These results suggest widespread mosaicism in founder generation transgenics.