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Analysis of protamines isolated from alcohol preserved epididymides
Author(s) -
Lee Catherine,
Mazrimas Joe,
Balhorn Rod
Publication year - 1991
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080300212
Subject(s) - protamine , sperm , biology , hamster , mesocricetus , alcohol , epididymis , andrology , polyacrylamide gel electrophoresis , biochemistry , microbiology and biotechnology , genetics , enzyme , medicine , heparin
Protamines 1 and 2 have been isolated from the sperm of frozen and isopropanol preserved Syrian hamster ( Mesocricetus auratus ) epididymides and analyzed by gel electrophoresis, high‐performance liquid chromatography (HPLC), and amino acid analysis and sequencing. The results show that alcohol preservation does not alter the primary structure of the two sperm nuclear proteins and that the preservation of mammalian reproductive organs in alcohol is a viable alternative to freezing tissues collected in the field. Sperm were isolated from tissues fixed in isopropanol for as long as 7 months without detectable adverse effects on either the isolation of sperm or the primary structure of the protamines.