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Unique 3′‐untranslated sequence of insulin‐like growth factor‐I isolated from human placenta
Author(s) -
Daimon M.,
Wang C.Y.,
Johnson T. R.,
Ilan Joseph,
Ilan Judith
Publication year - 1991
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080290305
Subject(s) - biology , untranslated region , clone (java method) , complementary dna , microbiology and biotechnology , three prime untranslated region , cdna library , nucleic acid sequence , northern blot , genetics , gene , messenger rna
A cDNA clone of 525 bp corresponding to the 3′‐untranslated region of insulin‐like growth factor‐I was isolated from a human placenta library. The sequence of this clone extended 200 nucleotides downstream from the previously reported 3′‐end of IGF‐IA cDNA, indicating the existence of IGF‐IA transcripts having an even larger 3′‐untranslated region. By using this clone for RNA transfer blot hybridization, it was shown that this longer 3′‐untranslated region is included in the 7.5‐ and 5.0‐kb transcripts, but not in the 1.1‐ and 0.9‐kb transcripts. It is also apparent that transcripts bearing the extended 3′‐untranslated sequence are highly expressed in human placenta.