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Induction of c‐ fos transcripts in early postimplantation mouse embryos by tgf‐α, EGF, PDGF, and FGF
Author(s) -
Nielsen Loretta L.,
Werb Zena,
Pedersen Roger A.
Publication year - 1991
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080290304
Subject(s) - biology , platelet derived growth factor receptor , fibroblast growth factor , transforming growth factor , epidermal growth factor , growth factor , embryo , platelet derived growth factor , microbiology and biotechnology , basic fibroblast growth factor , fibroblast growth factor receptor 3 , gene expression , messenger rna , transcription factor , fibroblast growth factor receptor 4 , receptor , fibroblast growth factor receptor , gene , genetics
The activity of growth factor receptors in the early postimplantation mouse embryo was studied by analyzing changes in expression of mRNA transcripts of an early response gene, c‐ fos , after binding of specific ligands. Reverse transcription of mRNA coupled with the polymerase chain reaction was used to detect gene transcription in single embryos after exposure to growth factors. Postimplantation embryos (at 7.5 days of gestation) had physiologically active receptors for transforming growth factor‐α (TGF‐α), epidermal growth factor (EGF), human platelet‐derived growth factor (PDGF), recombinant PDGF‐AA homodimer, and basic fibroblast growth factor (FGF), as indicated by induced expression of c‐ fos mRNA. c‐ fos expression was not induced in untreated embryos or in embryos incubated with active recombinant PDGF‐BB homodimer. These results show that growth factor receptors are functional during early mammalian embryogenesis.