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Cross‐linking a maturation‐dependent ram sperm plasma membrane antigen induces the acrosome reaction
Author(s) -
McKin Christine A.,
Weaver Frances E.,
Yoder Jeffrey A.,
Fairbanks Grant,
Wolf David E.
Publication year - 1991
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080290216
Subject(s) - acrosome reaction , acrosome , sialoglycoprotein , biology , sperm , antibody , andrology , epididymis , antigen , ionophore , microbiology and biotechnology , immunology , membrane , biochemistry , genetics , medicine
ESA152 is a highly hydrophobic 18 kDa sialoglycoprotein, which becomes expressed on ram sperm in the proximal cauda epididymis. ESA 152 is expressed on all regions of the sperm surface, most strongly on the posterior region of the head, most weakly on the anterior region of the head. In this paper, we show that induction of the acrosome reaction with Ca 2+ ionophore causes ESA152 to be redistributed from the posterior to the anterior region of the head plasma membrane. Cross‐linking ESA152 with bivalent antibody causes similar redistribution and induces the acrosome reaction, Induction of the acrosome reaction with ESA152 antibody requires Ca 2+ but is insensitive to (10 ng/ml) pertussis toxin.