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Association of exogenous DNA with cattle and insect spermatozoa in vitro
Author(s) -
Atkinson Peter W.,
Hines Eric R.,
Beaton Sandra,
Matthaei Klaus I.,
Reed Ken C.,
Bradley Mark P.
Publication year - 1991
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080290102
Subject(s) - biology , lucilia cuprina , sperm , dna , insect , exogenous dna , lucilia , in vitro , genetics , zoology , andrology , calliphoridae , botany , larva , medicine
Spermatozoa isolated from domestic cattle ( Bos taurus ), the Australian sheep blowfly ( Lucilia cuprina ) and the honeybee ( Apis mellifera ) are capable of binding exogenous radiolabeled linear DNA. Both motile and nonmotile bovine sperm exhibit four distinct patterns of DNA association. Following treatment with DNase I, the relative proportion of one of these patterns increases specifically in living sperm, suggesting that a small proportion of DNA that associates with bovine sperm may be sequestered within the sperm head.