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Identification of spectrin and calmodulin in rabbit spermiogenesis and spermatozoa
Author(s) -
Camatini Marina,
Colombo Anita,
Bonfanti Patrizia
Publication year - 1991
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080280110
Subject(s) - spectrin , biology , calmodulin , immunogold labelling , cytoskeleton , spermiogenesis , actin , microbiology and biotechnology , sperm , gene isoform , acrosome , ultrastructure , cell , biochemistry , anatomy , genetics , gene , enzyme
Actin was localized in the four subacrosomal bulges (Camatini et al., Eur. J. Cell Biol. 45:276–281, 1987), which characterize the sperm head of rabbit spermatozoa (Phillips, J. Ultrastruct. Res. 38:591–604, 1972), and in the postacrosomal region (Welch and O'Rand, Dev. Biol. 109:411–417, 1985; Camatini et al., Eur. J. Cell Biol. 45:276–281, 1987). Specific antibodies and indirect immunogold labelling on testis Lowicryl K4M sections and spermatozoa cryosections were used to study the distribution of calmodulin and a spectrin‐like protein. This protein was also present close to the shaping membranes of the head. The results presented suggest a membrane‐cytoskeletal role of actin, spectrin, and calmodulin.