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In vitro fertilization of horse follicular oocytes matured in vitro
Author(s) -
Zhang J. J.,
Muzs L. Z.,
Boyle M. S.
Publication year - 1990
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080260411
Subject(s) - in vitro , biology , andrology , bovine serum albumin , human fertilization , in vitro fertilisation , sperm , fertilisation , follicular fluid , follicular phase , in vitro maturation , horse , oocyte , anatomy , cryopreservation , biochemistry , endocrinology , embryo , reproductive technology , botany , medicine , microbiology and biotechnology , paleontology
In vitro fertilizing ability of stallion spermatozoa was assessed using horse follicular oocytes matured in vitro. After collection, stallion spermatozoa were either: 1) washed and incubated in TALP medium with 3 mg/ml bovine serum albumin (BSA) and 10 μg/ml heparin for 4 h, 2) washed and incubated in TALP with 3 mg/ml BSA for 3 h and cultured for a further 1 h with 1 mM caffeine and 5 mM dbcAMP, 3) washed and incubated in TALP medium with 3 mg/ml BSA at pH 7.9–8.2 for 2–4 h, or 4) diluted and incubated in TALP medium with 10 mg/ml BSA and 7.14 μM calcium ionophore A 23187 for 5–10 min followed by washing. After a given pretreatment, suspensions were diluted into B2 medium to a concentration of 5 × 10 6 sperm/ml and co‐incubated with oocytes for 12 h or 24–48 h. In the ionophoretreated group, 18 of 54 oocytes (33%) were fertilized by 12 h, and 11 of 45 (24%) cleaved by 24–48 h. Evidence of fertilization was not found in the oocytes incubated with spermatozoa from other treatment procedures.