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Dimethylsulphoxide affects the organisation of microfilaments in the mouse oocyte
Author(s) -
Vincent C.,
Pickering S. J.,
Johnson M. H.,
Quick S. J.
Publication year - 1990
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080260306
Subject(s) - biology , oocyte , microfilament , microbiology and biotechnology , andrology , genetics , embryo , cytoskeleton , cell , medicine
The effect of dimethylsulphoxide (DMSO) on microfilament organisation has been studied in the mouse oocyte after staining with (NBD)‐phallacidin. The cortical actin meshwork was disrupted by exposure of oocytes to 1.5 M DMSO at 37°C, and this disruption was associated with changes in the cell surface, especially microvilli length and distribution, as observed by scanning electron microscopy. The irregular distribution of actin filaments observed also appears to lead to an irregular expansion of the cell after DMSO removal. However, when exposure to DMSO was combined with cooling, the effects on the microfilament system were much reduced. The reversibility of DMSO action is considered and the potential implications of microfilament disruption on the viability and functions of the oocyte discussed.