Premium
Changes in protein phosphorylation associated with compaction of the mouse preimplantation embryo
Author(s) -
Bloom Theodora,
McConnell Josie
Publication year - 1990
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080260302
Subject(s) - biology , embryo , phosphorylation , microbiology and biotechnology , genetics
In order to investigate the role of protein phosphorylation in the early differentiative events of mouse preimplantation development, timed groups of embryos of various stages were incubated in medium containing [ 32 P]orthophosphate and harvested immediately after labelling or following a chase period. The phosphoproteins obtained were separated by electrophoresis in one and two dimensions. While some of the phosphoproteins found were common to all the stages examined, the detection of many depended on both the combination of pulse‐labelling and chase periods used and on the developmental stage examined. Some phosphoproteins were only found in compacted 8‐cell embryos, a correlation which suggests a possible link with the post‐translational mechanisms which underlie compaction.