Premium
Activation of cumulus‐free mouse oocytes
Author(s) -
Marcus G. J.
Publication year - 1990
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080260210
Subject(s) - biology , calcium , mouse strain , cloning (programming) , microbiology and biotechnology , ionophore , parthenogenesis , andrology , cumulus oophorus , embryo , medicine , oocyte , genetics , membrane , gene , computer science , programming language
Attempts were made to activate parthenogenetically oocytes from randombred mice under conditions required for nuclear transfer, viz. in the absence of cumulus cells. Of the large number of stimulants previously described and presumed to be generally effective, only exposure to strontium ion (as a replacement for calcium ion in the medium), or to calcium ionophore, activated a substantial proportion of cumulus‐free oocytes from TRf mice, a synthetic, random‐bred strain. These stimuli were less effective in oocytes from random‐bred ICR mice. The relevance of the findings to nuclear‐transfer cloning attempts in mice is discussed.