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Ca 2+ uptake during capacitation of mouse spermatozoa and the effect of an anion transport inhibitor on Ca 2+ uptake
Author(s) -
Ruknudin A.,
Silver I. A.
Publication year - 1990
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080260110
Subject(s) - capacitation , divalent , calcium , ionomycin , acrosome reaction , biology , ionophore , extracellular , human fertilization , sperm , biochemistry , nigericin , egta , biophysics , chemistry , membrane , anatomy , intracellular , botany , organic chemistry
With a specially constructed chamber, Ca 2+ uptake by mouse spermatozoa was monitored continuously during capacitation and the acrosome reaction. It was shown, using calcium ion‐selective microelectrodes, that there was an initial uptake of Ca 2+ by spermatozoa undergoing capacitation. Such net transport was also promoted by the divalent cation ionophores A23187 or ionomycin. An anion inhibitor, SITS, produced dose‐dependent inhibition of Ca 2+ uptake. This inhibitor reduced the incidence of capacitation as revealed by a reduction in the B pattern by chlortetracycline (CTC) assay and thus inhibited fertilization, suggesting that anions are involved in calcium uptake in mouse spermatozoa.