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Cytological effects of the microinjection of antibody to ras p21 in early cleavage Xenopus embryos
Author(s) -
Miron MarieJosé,
Lanoix Joël,
Paiement Jacques
Publication year - 1990
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080250403
Subject(s) - blastomere , cleavage (geology) , biology , xenopus , microbiology and biotechnology , microinjection , cytoplasm , chromatin , endoplasmic reticulum , mitosis , cleavage furrow , embryo , cytokinesis , cell , dna , embryogenesis , cell division , genetics , gene , paleontology , fracture (geology)
The presence of two ras ‐related proteins (22 and 23 kDa) was demonstrated in Xenopus embryonic extracts by selective immunoprecipitation using anti‐ ras monoclonal antibodies 142‐24EO5 and Y13‐259. We further describe the cytological effects of the microinijection of anti‐ ras monoclonal antibody Y13‐259 into early cleavage blastomeres of Xenopus embryos. Injection of the antibody into a blastomere at the two‐, four‐, or eight‐cell stage caused cleavage arrest in the descendants of the injected blastomere. Light microscopy (LM) of cleavage‐arrested cells revealed extensive deformation of the cells as well as heterogeneity of distribution of yolk platelets and pigment granules. LM analysis of serial sections of cleavage‐arrested cells revealed the presence of multiple nuclei. Although the nuclei expressed similar morphological properties, indicating that they were probably in the same stage of the nuclear cycle, they revealed highly variable chromatin densities. Electron microscope (EM) analysis of the cytoplasm of cleavage‐arrested cells revealed the accumulation of vesicles and large membranous elements coincident with cleavage arrest. Further‐more, endoplasmic reticulum (ER) existed in two forms, as closed, circular profiles and as long, linear arrays. Mitochondria were characteristically aligned in single file on both sides of the two types of ER cisternae. EM analysis of nuclei confirmed variations in chromatin organization and suggested the occurrence of unique nuclear envelope fusion among micronuclei in cleavage‐arrested cells. Cleavage arrest and changes in cytological features were not observed in the cytoplasm of cells microinjected with normal rat lgG. Thus the immunochemical data and microinjection experiments suggest that ras ‐like or ras antigenicity exists within rapidly replicating Xenopus blastomeres and may be involved in the organization of a number of its cytoplasmic elements.