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Gene transfer, expression and inheritance of PRSV‐rainbow trout‐GH cDNA in the common carp, Cyprinus carpio (Linnaeus)
Author(s) -
Zhang Peijung,
Hayat Mohammad,
Joyce Christopher,
GonzalezVillaseñor Lucia Irene,
Lin C. M.,
Dunham Rex A.,
Chen Thomas T.,
Powers Dennis A.
Publication year - 1990
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1080250103
Subject(s) - biology , rainbow trout , cyprinus , common carp , complementary dna , genetics , carp , gene , inheritance (genetic algorithm) , zebrafish , fishery , zoology , fish <actinopterygii>
A recombinant plasmid containing the Rous sarcoma virus‐long terminal repeat (RSV‐LTR) promoter linked to rainbow trout ( Salmo gairdneri ) growth hormone (GH) cDNA was microinjected into fertilized carp eggs. Genomic DNA extracted from pectoral fin of individual presumptive transgenic fish was analyzed by dot blot and Southern blot hybridization, using the RSV‐LTR and/or the GH cDNA sequences as probes. Out of 365 presumptive transgenic fish analyzed, 20 individuals were found to contain pRSV‐rtGH‐cDNA sequence in the genomic DNA. Expression of the trout GH polypeptide was detected by immunobinding assay in the red blood cells of nine transgenic fish tested. The level of expression, however, varied among the transgenics and could not be correlated with exogenous DNA copy number. Although there was considerable variation in the sizes of the transgenic fish, those microinjected during the one‐cell stage were (P < 0.05) 22% larger, on the average, than their sibling controls. A randomly selected fraction of the progeny derived from crosses between transgenic males and non‐transgenic females inherited the foreign DNA. These transgenic progeny grew faster (P < 0.05) than their non‐transgenic siblings.