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Cloning and characterization of a novel mouse immunoglobulin superfamily gene expressed in early spermatogenic cells
Author(s) -
Wakayama Tomohiko,
Ohashi Kazumasa,
Mizuno Kensaku,
Iseki Shoichi
Publication year - 2001
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1072
Subject(s) - biology , microbiology and biotechnology , messenger rna , epididymis , complementary dna , in situ hybridization , spermatogenesis , gene , signal peptide , antibody , immunoglobulin superfamily , testicle , gene expression , cdna library , cloning (programming) , peptide sequence , genetics , endocrinology , sperm , computer science , programming language
We cloned and characterized a novel immunoglobulin superfamily gene from the cDNA library of adult mouse testis. This gene was expressed in the spermatogenic cells and hence termed SgIGSF . The predicted SgIGSF protein was composed of 445 amino‐acid residues and contained a signal peptide, three extracellular immunoglobulin (Ig)‐like domains, a transmembranous domain, and a cytoplasmic domain. SgIGSF mRNA consisted of two size species, 2.1‐ and 4.5‐kb in length. Besides testis, SgIGSF mRNA was also expressed in a variety of organs, including the cerebrum, liver, kidney, and epididymis. The testis and liver expressed both the 2.1‐ and 4.5‐kb transcripts, whereas the cerebrum and epididymis predominantly expressed the 4.5‐kb one. In situ hybridization analysis in testis revealed that SgIGSF mRNA signal was localized to the spermatogenic cells from spermatogonia to zygotene spermatocytes. These results suggested that SgIGSF occurs in the plasma membrane of spermatogenic cells during the earlier stages of spermatogenesis. Mol. Reprod. Dev. 60: 158–164, 2001. © 2001 Wiley‐Liss, Inc.