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Comparison of intracytoplasmic sperm injection for inbred and hybrid mice
Author(s) -
Kawase Yosuke,
Iwata Takamitsu,
Toyoda Yutaka,
Wakayama Teruhiko,
Yanagimachi Ryuzo,
Suzuki Hiroshi
Publication year - 2001
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1063
Subject(s) - intracytoplasmic sperm injection , biology , embryo , human fertilization , oocyte , andrology , inbred strain , sperm , embryogenesis , c57bl/6 , oocyte activation , genetics , in vitro fertilisation , immunology , gene , medicine
We compared the results of intracytoplasmic sperm injection (ICSI) that leads to full term development of hybrid (B6C3F1 and B6D2F1) and inbred (C57BL/6) mouse embryos. Although fertilization and pre‐implantation development of C57BL/6 eggs were similar to those of F1 hybrid eggs, post‐implantation development of the embryos from C57BL/6 females was significantly poorer than those of the eggs from hybrid females. Reciprocal crosses of C57BL/6 and B6C3F1 gametes revealed that the low rate of post‐implantation development of C57BL/6 embryos was due to oocyte factor(s), rather than the sperm factor. Mol. Reprod. Dev. 60: 74–78, 2001. © 2001 Wiley‐Liss, Inc.