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Control of trophectoderm differentiation by inner cell mass‐derived fibroblast growth factor‐4 in mouse blastocysts and corrective effect of fgf‐4 on high glucose‐induced trophoblast disruption
Author(s) -
LeundaCasi Amaya,
de Hertogh René,
Pampfer Serge
Publication year - 2001
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.1059
Subject(s) - inner cell mass , trophoblast , biology , blastocyst , fibroblast growth factor , paracrine signalling , microbiology and biotechnology , immunology , endocrinology , embryo , placenta , embryogenesis , genetics , receptor , pregnancy , fetus
Abstract Previous studies have suggested that fibroblast growth factor‐4 (FGF‐4) may be a paracrine signal used by inner cell mass (ICM) cells to maintain adjacent trophectoderm (TE) cells in an undifferentiated state. In the present work, immunocytochemical analysis of mouse blastocysts confirmed that FGF‐4 was predominantly detected in the ICM before and after spreading over a fibronectin‐coated culture substrate. Addition of human recombinant FGF‐4 did not influence morphological progression, cell allocation and proliferation in ICM and TE lineages or mitosis and karyorhexis frequencies during blastocyst expansion. Addition of FGF‐4 to outgrowing blastocysts, in contrast, induced a significant decrease in the surface of the trophoblast outgrowths formed by the TE cells and in the proportion of giant trophoblasts per outgrowth. The fact that blastocysts display excessive trophoblast expansion and spreading over their culture substrate upon pre‐exposure to high concentrations of glucose in vitro was used to further assess the regulatory effect of FGF‐4. Addition of FGF‐4 was indeed found to fully neutralize the disruptive impact of high glucose on trophoblast outgrowths. Altogether, our data indicate that ICM‐derived FGF‐4 participates actively in the regulation of trophoblast development. Mol. Reprod. Dev. 60: 38–46, 2001. © 2001 Wiley‐Liss, Inc.