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Peroxisome proliferator‐activated receptor delta expression and regulation in mouse uterus during embryo implantation and decidualization
Author(s) -
Ding NaiZheng,
Teng ChunBo,
Ma Hong,
Ni Hua,
Ma XingHong,
Xu LiBin,
Yang ZengMing
Publication year - 2003
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.10348
Subject(s) - decidualization , biology , blastocyst , endocrinology , medicine , peroxisome proliferator activated receptor , embryo , estrogen , endometrium , decidua , estrogen receptor , receptor , embryogenesis , fetus , pregnancy , microbiology and biotechnology , placenta , genetics , cancer , breast cancer
The aim of this study was to examine the expression and regulation of peroxisome proliferator‐activated receptor (PPAR) PPARδ gene in mouse uterus during early pregnancy by in situ hybridization and immunohistochemistry. PPARδ expression under pseudopregnancy, delayed implantation, hormonal treatment, and artificial decidualization was also investigated. There was a very low level of PPARδ expression on days 1–4 of pregnancy. On day 5 when embryo implanted, PPARδ expression was exclusively observed in the subluminal stroma surrounding the implanting blastocyst. No corresponding signals were seen in the uterus on day 5 of pregnancy. There was no detectable PPARδ signal under delayed implantation. Once delayed implantation was terminated by estrogen treatment and embryo implanted, a strong level of PPARδ expression was induced in the subluminal stroma surrounding the implanting blastocyst. Estrogen treatment induced a moderate level of PPARδ expression in the glandular epithelium, while progesterone treatment had no effects in the ovariectomized mice. A strong level of PPARδ expression was seen in the decidua on days 6–8 of pregnancy. PPARδ expression was also induced under artificial decidualization. These data suggest that PPARδ expression at implantation sites require the presence of an active blastocyst and may play an essential role for blastocyst implantation. Mol. Reprod. Dev. 66: 218–224, 2003. © 2003 Wiley‐Liss, Inc.