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Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray
Author(s) -
Ishiwata Hiroko,
Katsuma Susumu,
Kizaki Keiichiro,
Patel Osman V.,
Nakano Hiroko,
Takahashi Toru,
Imai Kei,
Hirasawa Akira,
Shiojima Satoshi,
Ikawa Hiroshi,
Suzuki Yasuhito,
Tsujimoto Gozoh,
Izaike Yoshiaki,
Todoroki Junichi,
Hashizume Kazuyoshi
Publication year - 2003
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.10292
Subject(s) - biology , endometrium , placenta , complementary dna , microarray , gene , microarray analysis techniques , placental lactogen , gene expression , andrology , microbiology and biotechnology , genetics , pregnancy , endocrinology , fetus , medicine
Gene expression analysis comparing nonpregnant with pregnant bovine uteri, including placenta, was performed with a custom cDNA microarray containing 1,933 independent genes. These genes were classified into six categories according to biological function, as follows: cell and tissue structural dynamics (108 genes), intercellular communication (221), intracellular metabolism (265), cell cycle and apoptosis (26), regulation of gene expression (113), expressed sequence tag (EST) and function unknown (617), and uncomplemented genes (583 clones). This array possessed bovine placental/endometrial specificity, as it included many pregnancy‐specific molecules, such as pregnancy‐associated glycoprotein‐1 (PAGs), placental lactogen (PLs), and prolactin‐related protein‐1 (PRPs). A total of 77 genes were induced and 12 repressed in the placenta/endometrium. Our results point to a fundamental role for bovine placental‐specific genes such as PAGs, PLs, and PRPs, in implantation and placentogenesis, and document that cDNA microarray analysis from bovine placenta/endometrium is possible and is a specific tool for monitoring genome‐wide gene expression during the establishment and maintenance of pregnancy. Mol. Reprod. Dev. 65: 9–18, 2003. © 2003 Wiley‐Liss, Inc.