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Developmental failure of hybrid embryos generated by in vitro fertilization of water buffalo ( Bubalus bubalis ) oocyte with bovine spermatozoa
Author(s) -
Patil Shekhar,
Totey Satish
Publication year - 2003
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.10269
Subject(s) - biology , blastocyst , embryo , andrology , human fertilization , embryogenesis , blastomere , oocyte , zygote , in vitro fertilisation , genetics , medicine
The developmental potential of inter‐species hybrid embryos produced by in vitro fertilization of in vitro matured buffalo oocytes with bovine spermatozoa was studied with a view to investigate pre‐implantation embryo development and its gross morphology, early embryonic gene expression, and embryonic genome activation. Fertilization events with both buffalo and cattle spermatozoa were almost similar. Overall fertilization rate with cattle spermatozoa was 78.4% was not significantly different from that of buffalo spermatozoa (80.2%). Initial cleavage rate between buffalo and hybrid embryo was also similar, and there was no significant difference in their developmental rate till 8‐cell stage (26.0 ± 4.1 vs. 24.3 ± 4.8). However, only 5.3% of hybrid embryos developed into blastocyst stage compared to 21.7% in buffalo. mRNA phenotyping of insulin‐like growth factor family (Insulin, insulin receptor, IGF‐I, IGF‐I receptor, IGF‐II, and IGF‐II receptor) and glucose transporter isoforms (GLUT‐I, II, III, IV) in hybrid embryos clearly showed that these molecules were not expressed after 8‐cell stage onward. Similarly, as observed in buffalo embryos, incorporation of 35 S‐methionine and 3 H‐uridine could not be observed in hybrid embryos from 8‐cell stage onward. This suggests that the maternal‐zygotic genome activation did not occur in hybrid embryos. Differential staining also showed that the blastomere stopped dividing after 8‐cell stage. Collectively, these parameters clearly showed that there was developmental failure of hybrid embryos. Mol. Reprod. Dev. 64: 360–368, 2003. © 2003 Wiley‐Liss, Inc.