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Toxicogenomic effects of neonatal exposure to diethylstilbestrol on mouse testicular gene expression in the long term: A study using cDNA microarray analysis
Author(s) -
Adachi Tetsuya,
Komiyama Masatoshi,
Ono Yushin,
Koh KyuBom,
Sakurai Kenichi,
Shibayama Takako,
Kato Masaki,
Yoshikawa Tsutomu,
Seki Naohiko,
Iguchi Taisen,
Mori Chisato
Publication year - 2002
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.10178
Subject(s) - biology , complementary dna , microarray , gene expression , microarray analysis techniques , gene , gene expression profiling , real time polymerase chain reaction , diethylstilbestrol , microbiology and biotechnology , dna microarray , andrology , fetus , reverse transcription polymerase chain reaction , genetics , estrogen , medicine , pregnancy
We examined the effect of neonatal exposure to diethylstilbestrol (DES) on mouse testicular gene expression, using in‐house mouse fetus (day 14.5) cDNA microarrays. Newborn male ICR mice were exposed to DES (50 μg/mouse/day) from neonatal day 1 to 5. Differential expression was detected in 14 genes in 4‐week‐old (day 28) mouse testes by cDNA microarray analysis; 11 genes ( AI035263 , AU080565 , AU080361 , AU080678 , AI131681 , AU080631 , AA986882 , AI037066 , AA986537 , AI156816 , and AI596237 ) were up‐regulated and three genes ( AI131656 , AI118968 , and AI117606 ) were down‐regulated in DES‐treated mouse testes. Higher expression levels of the former eight genes, out of the up‐regulated genes picked‐up by the microarray, were also confirmed by reverse transcription and real‐time polymerase chain reaction (real‐time RT‐PCR) analysis. However, the differential expression of other genes could not be confirmed. Real‐time RT‐PCR analysis also revealed that expression levels of the eight genes were still higher in DES‐treated testes at 8 and 12 weeks of age. Our results suggest that cDNA microarray analysis is a useful method by which a large number of gene expressions are simultaneously detected and changes in gene expression are screened. In addition, our results suggest that these genes, whose expressions are changed in the testes of adult mice by fetal or neonatal exposure to exogenous chemicals, might be candidates for predictive biological markers. Mol. Reprod. Dev. 63: 17–23, 2002. © 2002 Wiley‐Liss, Inc.