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Immunological evidence for progesterone and estradiol receptors in the freshwater crayfish Austropotamobius pallipes
Author(s) -
Paolucci Marina,
Cristo Carlo Di,
Cosmo Anna Di
Publication year - 2002
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.10158
Subject(s) - biology , crayfish , receptor , crustacean , decapoda , zoology , ecology , fishery , genetics
Abstract In this article, we present evidence for progesterone and estradiol receptors (PR and ER, respectively) in the female of the crayfish Austropotamobius pallipes . To our knowledge, this is the first report of sex steroid receptors in crustaceans. By using immunohistochemistry and Western blotting approaches and employing three different antibodies against PR (human PR, chicken PR‐hinge region, and chicken PR A/B domain) and antibodies against human ER, we showed the presence of PR in the ovary and hepatopancreas and ER in the hepatopancreas of the freshwater crayfish A. pallipes . The immunological characteristics and the tissue localization suggest a relatedness with both PR and ER in vertebrates along with their involvement in the modulation of reproductive functions in this crustaceans. Mol. Reprod. Dev. 63: 55–62, 2002. © 2002 Wiley‐Liss, Inc.