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Modern proton‐detected 1D 1 H– 15 N NMR experiments. Application to the measurement of 1 H, 15 N coupling constants at natural abundance
Author(s) -
Parella Teodor,
Belloc Jordi
Publication year - 2002
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.977
Subject(s) - chemistry , coupling constant , proton , nuclear magnetic resonance spectroscopy , proton nmr , spectral line , abundance (ecology) , coupling (piping) , transverse relaxation optimized spectroscopy , carbon 13 nmr satellite , two dimensional nuclear magnetic resonance spectroscopy , pulse sequence , analytical chemistry (journal) , nuclear magnetic resonance , fluorine 19 nmr , stereochemistry , organic chemistry , nuclear physics , physics , mechanical engineering , particle physics , astronomy , fishery , engineering , biology
Abstract Non‐selective and selective versions of several proton‐detected 1D NMR experiments to be applied to 15 N are proposed. Clean, artifact‐free 1D spectra are easily obtained by the effective coherence selection by pulsed‐field gradients and the attainable sensitivity is maximized using modern pulse schemes. Despite the low sensitivity inherent to 15 N NMR spectroscopy, the successful application of these experiments is demonstrated for resonance assignments and accurate measurement of both one‐bond and long‐range proton–nitrogen coupling constants on a model tripeptide at natural abundance. Copyright © 2001 John Wiley & Sons, Ltd.