Clean adiabatic TOCSYs

Various adiabatic mixing schemes are explored using numerical simulations with the aim of finding suitable mixing sequences for fast rotating samples with relatively wide resonances and poorly resolved J ‐couplings, such as proteins or resin‐bound products of solid‐phase synthesis. It is shown both experimentally and by simulations that WURST‐2 mixing scheme provides the best sensitivity and the lowest interference from cross‐relaxation effects in the spin diffusion limit. For medium‐sized molecules, WURST‐1, constant adiabaticity Gaussian and hyperbolic secant are the possible alternatives. Copyright © 2001 John Wiley & Sons, Ltd.