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Deuterium solid‐state NMR quadrupolar order rotating frame relaxation with applications to amyloid‐β fibrils
Author(s) -
Vugmeyster Liliya,
Ostrovsky Dmitry
Publication year - 2020
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.5114
Subject(s) - chemistry , solid state nuclear magnetic resonance , deuterium , coherence (philosophical gambling strategy) , relaxation (psychology) , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , molecular physics , atomic physics , physics , stereochemistry , quantum mechanics , psychology , social psychology
We describe a new method for measuring molecular dynamics based on the deuterium solid‐state nuclear magnetic resonance (NMR) quadrupolar order rotating frame relaxation rate R 1 ρ ,Q under static conditions. The observed quadrupolar order coherence is created using the broad‐band Jeener–Broekaert excitation and is locked with a weak radio frequency (RF) field. We describe the experimental and theoretical approaches and show applications to a selectively deuterated valine side chain of the phosphorylated amyloid‐β (1–40) fibrils phosphorylated at the serine‐8 position. The R 1 ρ ,Q rate is sensitive to the rotameric exchange mode. For biological samples, the low spin‐lock field in the 5‐ to 10‐kHz range has the advantage of avoiding sample heating and dehydration. Thus, it provides an alternative to approaches based on single‐quantum coherence, which require larger spin‐lock fields.