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Optimal flip angle for robust practical quantitative NMR measurement using a fixed pulse length
Author(s) -
Gao Jinhai,
Tsao Jeffrey,
Rajan Srinivasan,
Wang Bing
Publication year - 2019
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4859
Subject(s) - calibration , chemistry , pulse (music) , spectrometer , flip angle , sample (material) , range (aeronautics) , analytical chemistry (journal) , quantitative analysis (chemistry) , accuracy and precision , biological system , computational physics , optics , physics , chromatography , statistics , materials science , mathematics , detector , medicine , biology , magnetic resonance imaging , composite material , radiology
NMR quantification has been traditionally performed by using internal standards. Although methods using external reference in NMR quantification have been developed, the major obstacles in using external referencing method are the measurement deviations associated with changing sample conditions and the requirement of pulse width calibration for every sample in order to compensate these errors. The calibration process is time consuming and in some cases impossible. We developed a quantitative NMR method fixed pulse length (FIXPUL) for all measurements without sample‐by‐sample calibration. The method is based on the use of an optimal flip angle calibrated for an external standard so that the quantitative errors associated with the pulse width variations are minimized. FIXPUL can be implemented on most basic NMR spectrometers and is robust and easily automated. The method is applicable to a wide range of solution NMR samples in chemistry, biology, and drug research and discovery.