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In vivo comprehensive multiphase NMR
Author(s) -
Liaghati Mobarhan Yalda,
Soong Ronald,
Lane Daniel,
Simpson Andre J.
Publication year - 2020
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4832
Subject(s) - magic angle spinning , chemistry , in vivo , spinning , proton nmr , nanotechnology , solid state nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , nuclear magnetic resonance , materials science , chromatography , physics , organic chemistry , microbiology and biotechnology , polymer chemistry , biology
Abstract Traditionally, due to different hardware requirements, nuclear magnetic resonance (NMR) has developed as two separate fields: one dealing with solids, and one with solutions. Comprehensive multiphase (CMP) NMR combines all electronics and hardware (magic angle spinning [MAS], gradients, high power Radio Frequency (RF) handling, lock, susceptibility matching) into a universal probe that permits a comprehensive study of all phases (i.e., liquid, gel‐like, semisolid, and solid), in intact samples. When applied in vivo, it provides unique insight into the wide array of bonds in a living system from the most mobile liquids (blood, fluids) through gels (muscle, tissues) to the most rigid (exoskeleton, shell). In this tutorial, the practical aspects of in vivo CMP NMR are discussed including: handling the organisms, rotor preparation, sample spinning, water suppression, editing experiments, and finishes with a brief look at the potential of other heteronuclei ( 2 H, 15 N, 19 F, 31 P) for in vivo research. The tutorial is aimed as a general resource for researchers interested in developing and applying MAS‐based approaches to living organisms. Although the focus here is CMP NMR, many of the approaches can be adapted (or directly applied) using conventional high‐resolution magic angle spinning, and in some cases, even standard solid‐state NMR probes.