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Practical aspects of real‐time pure shift HSQC experiments
Author(s) -
Kiraly Peter,
Nilsson Mathias,
Morris Gareth A.
Publication year - 2018
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4704
Subject(s) - homonuclear molecule , heteronuclear molecule , heteronuclear single quantum coherence spectroscopy , chemistry , context (archaeology) , time shifting , free induction decay , resolution (logic) , nuclear magnetic resonance , pulse sequence , paradigm shift , computational physics , nuclear magnetic resonance spectroscopy , computer science , physics , molecule , artificial intelligence , quantum mechanics , medicine , paleontology , telecommunications , organic chemistry , spin echo , radiology , transmission (telecommunications) , magnetic resonance imaging , biology
Pure shift NMR spectroscopy has become an efficient tool for improving resolution in proton NMR spectra by removing the effect of homonuclear couplings. The introduction of real‐time acquisition methods has allowed the main drawback of pure shift NMR, the long experiment times needed, to be circumvented. Real‐time methods use periodic application of J ‐refocusing pulse sequence elements, acquiring a single free induction decay, in contrast to previous methods that construct a pure shift interferogram by concatenating excerpts from multiple free induction decays. In the important heteronuclear single‐quantum correlation experiment, implementing real‐time pure shift data acquisition typically leads to the simultaneous improvement of both resolution and sensitivity. The current limitations of and problems with real‐time pure shift acquisition methods are discussed here in the context of heteronuclear single‐quantum correlation experiments. We aim to provide a detailed account of the technical challenges, together with a practical guide to exploiting the full potential of such methods.