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Extracting unresolved coupling constants from complex multiplets by a real‐time J ‐upscaled SERF experiment
Author(s) -
Buchberger Kathrin,
Walenta Martin,
Zangger Klaus
Publication year - 2018
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4699
Subject(s) - homonuclear molecule , chemistry , coupling constant , j coupling , scalar (mathematics) , signal (programming language) , excitation , coupling (piping) , resolution (logic) , spectral line , nuclear magnetic resonance , molecular physics , analytical chemistry (journal) , computational physics , nuclear magnetic resonance spectroscopy , physics , molecule , quantum mechanics , stereochemistry , geometry , mechanical engineering , mathematics , organic chemistry , chromatography , artificial intelligence , computer science , engineering , programming language
The measurement of small homonuclear coupling constants is often prevented by either their small size and/or overlap with other signal splittings. Here, we present a real‐time method to extract such couplings without interference from other splittings, with a resolution that is beyond conventional NMR spectra. In this real‐time J ‐upscaled SERF experiment, homonuclear coupling is removed by slice‐selective pure shift NMR, whereas scalar coupling to only one selected signal is reintroduced by selective refocusing. The remaining couplings are enhanced by real‐time J ‐upscaling during interruptions of the FID data acquisition. The resulting spectrum is not only simplified by the restriction of the scalar coupling but also its resolution enhanced. This improved resolution results from a reduction of signal broadening due to magnetic field inhomogeneities from 2 different sources: slice‐selective excitation and the spin‐echo type J ‐upscaling element.