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Homonuclear decoupling in the 13 C indirect dimension of HSQC experiments for 13 C‐enriched compounds
Author(s) -
Brucka Marta,
Sheberstov Kirill F.,
Jeannerat Damien
Publication year - 2018
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4697
Subject(s) - homonuclear molecule , chemistry , heteronuclear single quantum coherence spectroscopy , decoupling (probability) , spectral line , nuclear magnetic resonance , heteronuclear molecule , two dimensional nuclear magnetic resonance spectroscopy , molecular physics , nuclear magnetic resonance spectroscopy , physics , stereochemistry , quantum mechanics , molecule , organic chemistry , control engineering , engineering
The two most compelling methods for broadband homonuclear decoupling currently available, Zangger–Sterk (ZS) and pure shift yielded by chirp excitation (PSYCHE), were successfully adapted and tested on the 13 C isotope. When applied during the indirect carbon evolution in the HSQC experiment, they both entirely eliminated the extended carbon–carbon multiplet structures observed in this dimension of a non‐decoupled HSQC spectrum of 13 C‐enriched cholesterol. The optimized selective pulse modulated using novel non‐equidistant scheme for multisite refocusing (ZS) and the small flip angle saltire chirps (PSYCHE) both proved to be robust and efficient in providing decoupled spectra with a sensitivity of about 25% that of the non‐decoupled HSQC spectra with improved quality compared to earlier results.