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Metabolic effects of Hedyotis diffusa on rats bearing W alker 256 tumor revealed by NMR ‐based metabolomics
Author(s) -
Wang Zhiyong,
Gao Kuo,
Xu Can,
Gao Jian,
Yan Yujing,
Wang Yingfeng,
Li Zhongfeng,
Chen Jianxin
Publication year - 2018
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4658
Subject(s) - metabolomics , chemistry , glutamine , creatinine , pharmacology , traditional chinese medicine , urine , choline , metabolite , biochemistry , chromatography , amino acid , medicine , pathology , alternative medicine
Hedyotis diffusa , a traditional Chinese herbal medicine, is widely used for oncotherapy and shows a positive effect in the clinical treatment. But its mechanism of anticancer activities is complicated and unclear. This study was undertaken to assess the therapeutic effects and reveal detailed mechanisms of H . diffusa for oncotherapy. A Walker 256 tumor‐bearing rat model was established, and metabolomic profiles of plasma and urine were obtained from 1 H NMR technique. Multivariate statistical analysis methods were used to characterize the discriminating metabolites between control (C), Walker 256 tumor‐bearing rats model (M), and H . diffusa treatment (H) groups. Finally, 13 and 10 metabolomic biomarkers in urine and plasma samples were further identified as characteristic metabolites in M group, whereas H group showed a partial metabolic balance recovered, such as ornithine, N ‐acetyl‐ l ‐aspartate, l ‐aspartate, and creatinine in urine samples, and acetate, lactate, choline, l ‐glutamine, and 3‐hydroxybutyrate in plasma samples. On the basis of the methods above, we hypothesized H . diffusa treatment reduced the injury caused by Walker 256 tumor and maintained a metabolic balance. Our study demonstrated that this method provided new insights into metabolic alterations in tumor‐bearing biosystems and researching on the effects of H . diffusa on the endogenous metabolism in tumor‐bearing rats.