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NMR line‐fitting quantification of polysaccharide N‐ acylurea‐based modification in glycoconjugates of Salmonella Typhi Vi polysaccharide
Author(s) -
Garrido Raine,
Soubal Jean Pierre,
Torres Leonid,
Ramírez Ubel,
Vérez Vicente
Publication year - 2017
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4579
Subject(s) - carbodiimide , chemistry , polysaccharide , glycoconjugate , adduct , dendrimer , combinatorial chemistry , organic chemistry , biochemistry
The polysaccharides modification via carbodiimide reaction is one of the most applied methods for obtaining conjugated vaccines against Salmonella enterica. However, N ‐acylurea carbodiimide adduct generated in the process is a critical impurity in carbohydrate‐based vaccines. A quantitative NMR method was developed for assessing the N ‐acylurea carbodiimide adduct impurity. The procedure was based on line‐fitting facilities for processing the NMR signals on complex spectra. The method showed good linearity, accuracy and precision under inter‐operator variation (relative standard deviation <5%). Copyright © 2017 John Wiley & Sons, Ltd.