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The new face of isotopic NMR at natural abundance
Author(s) -
Jézéquel Tangi,
Joubert Valentin,
Giraudeau Patrick,
Remaud Gérald S.,
Akoka Serge
Publication year - 2017
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4548
Subject(s) - chemistry , isotope , natural abundance , abundance (ecology) , isotope ratio mass spectrometry , isotope fractionation , isotopic signature , isotope analysis , analytical chemistry (journal) , mass spectrometry , fractionation , environmental chemistry , nuclear physics , chromatography , physics , geology , oceanography , fishery , biology
The most widely used method for isotope analysis at natural abundance is isotope ratio monitoring by Mass Spectrometry (irm‐MS) which provides bulk isotopic composition in 2 H, 13 C, 15 N, 18 O or 34 S. However, in the 1980s, the direct access to Site‐specific Natural Isotope Fractionation by Nuclear Magnetic Resonance (SNIF‐NMR TM ) was immediately recognized as a powerful technique to authenticate the origin of natural or synthetic products. The initial – and still most popular – application consisted in detecting the chaptalization of wines by irm‐ 2 H NMR. The approach has been extended to a wide range of methodologies over the last decade, paving the way to a wide range of applications, not only in the field of authentication but also to study metabolism. In particular, the emerging irm‐ 13 C NMR approach delivers direct access to position‐specific 13 C isotope content at natural abundance. After highlighting the application scope of irm‐NMR ( 2 H and 13 C), this article describes the major improvements which made possible to reach the required accuracy of 1‰ (0.1%) in irm‐ 13 C NMR. The last part of the manuscript summarizes the different steps to perform isotope analysis as a function of the sample properties (concentration, peak overlap) and the kind of targeted isotopic information (authentication, affiliation). Copyright © 2016 John Wiley & Sons, Ltd.