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NMR artifacts caused by decoupling of multiple‐spin coherences: improved SLAP experiment
Author(s) -
Blechta Vratislav,
Schraml Jan
Publication year - 2015
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4221
Subject(s) - chemistry , decoupling (probability) , pulse sequence , polarization (electrochemistry) , isotopomers , spin (aerodynamics) , nuclear magnetic resonance , molecule , physics , organic chemistry , control engineering , engineering , thermodynamics
Contrary to common expectations, multiple‐spin coherences containing products of proton and heteronucleus operators (e.g. H u C x , u = x , y , z ) can produce not only sidebands but also noticeable centerband NMR signals of the heteronucleus during acquisition under 1 H broadband decoupling. Such centerband signals of low abundant heteronuclei can be sources of relatively strong unexpected artifacts in NMR experiments that aim to detect very weak signals from much less‐abundant isotopomers, e.g. 13 C– 13 C ones. These findings lead to a new design of Sign Labeled Polarization Transfer (SLAP) pulse sequence (MSS‐SLAP) with improved suppression of centerband peaks that are because of singly, e.g. 13 C, labeled molecules (parent peaks). The MSS‐SLAP experiment and its MSS‐BIRD‐SLAP variant are compared with a few older SLAP versions. Copyright © 2015 John Wiley & Sons, Ltd.