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HOBS methods for enhancing resolution and sensitivity in small DNA oligonucleotide NMR studies
Author(s) -
McKenna Josiah M.,
Parkinson John A.
Publication year - 2015
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4182
Subject(s) - homonuclear molecule , chemistry , two dimensional nuclear magnetic resonance spectroscopy , proton nmr , sensitivity (control systems) , nuclear overhauser effect , resolution (logic) , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , molecule , stereochemistry , physics , organic chemistry , artificial intelligence , electronic engineering , computer science , engineering
1 H NMR spectra from biopolymers give chemical shifts classified according to proton type and often suffer from signal degeneracy. Data from nucleic acids are particularly prone to this failing. Recent developments in proton broadband decoupling techniques with the promise of enhanced resolution at full sensitivity have allowed us to investigate the application of homonuclear band‐selective (HOBS) decoupling to the study of small synthetic DNA molecules and to compare these with results from classical and pure shift techniques. Improved signal resolution at full sensitivity in both HOBS‐1D 1 H and HOBS‐2D [ 1 H, 1 H] NOESY NMR data is reported for three example small DNA molecules. Comparisons of 1 H T 1 and integrals of signals from HOBS‐1D 1 H and HOBS‐2D [ 1 H, 1 H] NOESY NMR data with those of standard data collection methods are also reported. The results show that homonuclear HOBS‐NOESY data are useful for data assignment purposes and have some merit for quantification purposes. In general, we show that resolution and sensitivity enhancement of 1 H NMR data for small DNA samples may be achieved without recourse to higher magnetic field strength at full sensitivity in a band‐selected manner. Copyright © 2014 John Wiley & Sons, Ltd.