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Suppression of phase and amplitude J(HH) modulations in HSQC experiments
Author(s) -
Castañar Laura,
Sistaré Eduard,
Virgili Albert,
Williamson R. Thomas,
Parella Teodor
Publication year - 2015
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4149
Subject(s) - heteronuclear single quantum coherence spectroscopy , chemistry , amplitude , multiplet , proton , phase (matter) , spins , nuclear magnetic resonance , spectral line , two dimensional nuclear magnetic resonance spectroscopy , physics , optics , nuclear physics , stereochemistry , quantum mechanics , condensed matter physics , organic chemistry
The amplitude and the phase of cross peaks in conventional 2D HSQC experiments are modulated by both proton–proton, J(HH), and proton–carbon, 1 J(CH), coupling constants. It is shown by spectral simulation and experimentally that J(HH) interferences are suppressed in a novel perfect‐HSQC pulse scheme that incorporates perfect‐echo INEPT periods. The improved 2D spectra afford pure in‐phase cross peaks with respect to 1 J(CH) and J(HH), irrespective of the experiment delay optimization. In addition, peak volumes are not attenuated by the influence of J(HH), rendering practical issues such as phase correction, multiplet analysis, and signal integration more appropriate. Copyright © 2014 John Wiley & Sons, Ltd.