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Molecular signal suppression by in situ microextraction in nuclear magnetic resonance spectroscopy
Author(s) -
Chandran Jima,
Shintu Laetitia,
Caldarelli Stefano
Publication year - 2014
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4120
Subject(s) - chemistry , in situ , molecule , nuclear magnetic resonance spectroscopy , polymer , bisphenol a , analytical chemistry (journal) , characterization (materials science) , signal (programming language) , molecularly imprinted polymer , two dimensional nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , chromatography , organic chemistry , nanotechnology , selectivity , stereochemistry , materials science , physics , computer science , epoxy , programming language , catalysis
The detailed characterization of complex mixtures by NMR is often hampered by the presence of signals from uninformative compounds, the resonances of which overlap with those of the molecules of interest. We provide here a proof of principle for an approach to NMR signal suppression in complex samples using Molecularly Imprinted Polymers (MIPS). Addition of a few milligrams of polymer to a solution traps the target molecule in typical micromolar to millimolar concentration, thus achieving in situ signal suppression, without altering any other spectral features. This method minimized any manipulation or perturbation of the spectrum and was applied to a complex mixture of known compounds and to a plant extract, in both cases spiked with a compound (bisphenol A), which was subsequently removed by selective binding to a complementary MIP. What is described in this report is comparable with microextraction and may in due course be applied to a large number of analytical challenges. Copyright © 2014 John Wiley & Sons, Ltd.