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Quantification of taxanes in a leaf and twig extract from Taxus baccata L. using 13 C NMR spectroscopy
Author(s) -
Paoli Mathieu,
Bighelli Ange,
Castola Vincent,
Tomi Félix,
Casanova Joseph
Publication year - 2013
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.4006
Subject(s) - taxus , chemistry , twig , taxane , diterpene , reproducibility , chromatography , nuclear magnetic resonance spectroscopy , stereochemistry , botany , medicine , cancer , breast cancer , biology
In the course of our ongoing work on the chemical characterization of Taxus baccata L. growing wild in Corsica, we have developed and validated a method for direct quantification of taxane derivatives by 13 C NMR using 10‐deacetylbaccatin III as reference compound and 1,6‐hexanediol as internal standard. We have observed good accuracy (relative errors between 0.3% and 3.5%), linearity ( R 2 = 0.999) and precision (reproducibility 8.5 mg ± 1.1%) of the measurements. The experimental procedure was applied to the quantification of six identified taxanes in a fraction of chromatography of a methanol extract of T . baccata leaves. This method can be applied to other compounds bearing the taxane skeleton. Copyright © 2013 John Wiley & Sons, Ltd.