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Systematic assignment of NMR spectra of 5‐substituted‐4‐thiopyrimidine nucleosides
Author(s) -
Zhang Xiaohui,
Wang Jian,
Xu YaoZhong
Publication year - 2013
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.3980
Subject(s) - chemistry , nucleobase , nuclear magnetic resonance spectroscopy , nucleotide , characterization (materials science) , proton nmr , stereochemistry , nuclear magnetic resonance spectroscopy of nucleic acids , nmr spectra database , nucleoside , two dimensional nuclear magnetic resonance spectroscopy , fluorine 19 nmr , spectral line , combinatorial chemistry , computational chemistry , transverse relaxation optimized spectroscopy , dna , nanotechnology , biochemistry , materials science , physics , astronomy , gene
Unambiguous characterization of 5‐substituted‐4‐thiopyrimidine nucleosides (ribonucleosides and 2'‐deoxynucleosides) was performed using NMR spectroscopy. Assignments of all proton and carbon signals of 5‐bromo‐4‐thiouridine and related nucleosides were systematically carried out and firmly established by COSY and HMQC techniques. The NMR data of various 4‐thiopyrimidine nucleosides are compared, and the key contributing factors discussed. The approach presented here is applicable to other modified nucleosides and nucleotides, as well as nucleobases. Copyright © 2013 John Wiley & Sons, Ltd.