Determination of  3  J ( 1 H3′ 31 P) couplings in a DNA oligomer with enhanced sensitivity employing a constant‐time TOCSY difference experiment | Zendy

Reith Lorenz M. | Zendy; Schlagnitweit Judith | Zendy; Smrecki Vilko | Zendy; Knör Günther | Zendy; Müller Norbert | Zendy; Schoefberger Wolfgang | Zendy

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Determination of 3 J ( 1 H3′ 31 P) couplings in a DNA oligomer with enhanced sensitivity employing a constant‐time TOCSY difference experiment

Author(s) -

Reith Lorenz M.,

Schlagnitweit Judith,

Smrecki Vilko,

Knör Günther,

Müller Norbert,

Schoefberger Wolfgang

Publication year - 2011

Publication title -

magnetic resonance in chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.483

H-Index - 72

eISSN - 1097-458X

pISSN - 0749-1581

DOI - 10.1002/mrc.2729

Subject(s) - chemistry , coupling constant , analytical chemistry (journal) , decoupling (probability) , oligomer , histone octamer , observable , amplitude , isotope , oligonucleotide , constant (computer programming) , pi , molecular physics , atomic physics , dna , physics , nuclear physics , quantum mechanics , chromatography , biochemistry , programming language , organic chemistry , control engineering , nucleosome , computer science , engineering , histone

A constant‐time TOCSY difference experiment for the determination of 3 J ( 1 H3′ 31 P) coupling constants in non‐isotope‐labelled DNA oligonucleotides is presented. The method is tested on a DNA octamer and compared with the established constant‐time NOESY difference method. Each 3 J ( 1 H3′ 31 P) coupling constant is determined from amplitude changes caused by phosphorous decoupling, which are observable on multiple cross‐peaks, thus leading to a high accuracy of the value of the 3 J ( 1 H3′ 31 P) coupling constant. The new experiment delivers up to three times the sensitivity compared with previously reported methods. Copyright © 2011 John Wiley & Sons, Ltd.

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