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A capacitively coupled temperature‐jump arrangement for high‐resolution biomolecular NMR
Author(s) -
Gal Maayan,
Zibzener Koby,
Frydman Lucio
Publication year - 2010
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.2675
Subject(s) - chemistry , liquid crystal , anisotropy , isotropy , electromagnetic coil , nuclear magnetic resonance spectroscopy , proton nmr , analytical chemistry (journal) , nuclear magnetic resonance , relaxation (psychology) , transverse relaxation optimized spectroscopy , optoelectronics , fluorine 19 nmr , optics , materials science , physics , electrical engineering , organic chemistry , chromatography , stereochemistry , engineering , psychology , social psychology
Abstract A simple design for performing rapid temperature jumps within a high‐resolution nuclear magnetic resonance (NMR) setting is presented and exemplified. The design is based on mounting, around a conventional NMR glass tube, an inductive radiofrequency (RF) irradiation coil that is suitably tuned by a resonant circuit and is driven by one of the NMR's console high‐power RF amplifiers. The electric fields generated by this coil's thin metal strips can lead to a fast and efficient heating of the sample, amounting to temperature jumps of ≈ 20 °C in well within a second—particularly in the presence of lossy dielectric media like those provided by physiological buffers. Moreover, when wound around a 4‐mm NMR tube, the resulting device fits a conventional 5‐mm inverse probe and is wholly compatible with the field homogeneities and sensitivities expected for high‐resolution biomolecular NMR conditions. The performance characteristics of this new system were tested using saline solutions, as well as on a lyotropic liquid crystal capable of undergoing nematic → isotropic transitions in the neighborhood of ambient temperature. These settings were then incorporated into the performance of a new kind of single‐scan 2D NMR spectroscopy acquisition, correlating the anisotropic and isotropic patterns elicited by solutes dissolved in such liquid‐crystalline systems, before and after a sudden temperature jump occurring during an intervening mixing period. Copyright © 2010 John Wiley & Sons, Ltd.