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Selective J ‐resolved HMBC, an efficient method for measuring heteronuclear long‐range coupling constants
Author(s) -
Furihata Kazuo,
Tashiro Mitsuru,
Seto Haruo
Publication year - 2009
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.2496
Subject(s) - heteronuclear molecule , pulse sequence , chemistry , pulse (music) , coupling constant , range (aeronautics) , sequence (biology) , analytical chemistry (journal) , coupling (piping) , nuclear magnetic resonance , stereochemistry , nuclear magnetic resonance spectroscopy , chromatography , physics , materials science , optics , biochemistry , particle physics , detector , metallurgy , composite material
An efficient pulse sequence for measuring long‐range CH coupling constants ( J CH ) named selective J ‐resolved HMBC has been developed by replacing a 1 H 180° pulse with a selective 1 H 180° pulse and the HMBC pulse scheme with the constant time (CT) HMBC employed in the J ‐resolved HMBC pulse sequence that we reported previously. The novel pulse sequence providing only long‐range J CH cross peaks for easy and accurate analysis enables to overcome disadvantages of the previous HMBC‐based pulse sequences ( J ‐resolved HMBC‐1) along with maintaining high sensitivity. The efficiency of measuring long‐range J CH using the proposed pulse sequence has been demonstrated in applications to the complicated natural products, portmicin and monazomycin. Copyright © 2009 John Wiley & Sons, Ltd.