Premium
Characterization of the complex formation of 1,6‐anhydro‐β‐maltotriose with potassium using 1 H and 39 K NMR spectroscopy
Author(s) -
Kato Takayuki,
Tsubono Koji,
Kamo Osamu,
Kato Toshiyo,
Furihata Kazuo,
Fujimoto Takashi,
Machinami Tomoya,
Tashiro Mitsuru
Publication year - 2009
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.2495
Subject(s) - maltotriose , chemistry , potassium , analytical chemistry (journal) , relaxation (psychology) , ion , diffusion , nuclear magnetic resonance spectroscopy , crystallography , stereochemistry , maltose , chromatography , organic chemistry , thermodynamics , psychology , social psychology , physics , enzyme
The 1 H and 39 K longitudinal relaxation times ( T 1 ) and 1 H diffusion coefficients were measured to investigate the complex formation of 1,6‐anhydro‐β‐maltotriose and potassium ions. Although the 1 H‐ T 1 values of H3′, H5′, H1″ and H4″ decreased in the presence of potassium ions, 1 H chemical shifts and 1 H diffusion coefficients did not show significant changes. The long‐range coupling constants of 3 J C−H around the glycosyl bonds did not show significant changes either. In the measurements of 39 K spectra, the 39 K signal obviously broadened and the 39 K‐ T 1 values decreased in the presence of 1,6‐anhydro‐β‐maltotriose, indicating the complex formation of 1,6‐anhydro‐β‐maltotriose and potassium ions. These results indicate that the conformation and molecular volume were unaffected in the complex formation. Copyright © 2009 John Wiley & Sons, Ltd.