Standard operating procedure for metabonomics studies of blood serum and plasma samples using a  1 H‐NMR micro‐flow probe | Zendy

Sukumaran Dinesh K. | Zendy; Garcia Erwin | Zendy; Hua Jia | Zendy; Tabaczynski Walter | Zendy; Odunsi Kunle | Zendy; Andrews Chris | Zendy; Szyperski Thomas | Zendy

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Standard operating procedure for metabonomics studies of blood serum and plasma samples using a 1 H‐NMR micro‐flow probe

Author(s) -

Sukumaran Dinesh K.,

Garcia Erwin,

Hua Jia,

Tabaczynski Walter,

Odunsi Kunle,

Andrews Chris,

Szyperski Thomas

Publication year - 2009

Publication title -

magnetic resonance in chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.483

H-Index - 72

eISSN - 1097-458X

pISSN - 0749-1581

DOI - 10.1002/mrc.2469

Subject(s) - chemistry , analytical chemistry (journal) , spectrometer , protocol (science) , multivariate statistics , proton nmr , data collection , nuclear magnetic resonance spectroscopy , chromatography , statistics , organic chemistry , mathematics , medicine , physics , alternative medicine , quantum mechanics , pathology

A standard operating procedure (SOP) is presented for high‐throughput metabonomics studies using a 600 MHz NMR spectrometer equipped with a micro‐flow probe that is connected to an auto‐sampler. The procedure is designed to minimize random and systematic variation of NMR data collection. In addition, a protocol is described to assess the quality of the data acquired by a given NMR spectroscopist to ensure that (i) all researchers involved in the NMR data acquisition of a metabonomics research program perform equally and (ii) operator‐associated variation of NMR data collection is statistically not relevant for the interpretation of results obtained from multivariate data analyses. Copyright © 2009 John Wiley & Sons, Ltd.

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