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Standard operating procedure for metabonomics studies of blood serum and plasma samples using a 1 H‐NMR micro‐flow probe
Author(s) -
Sukumaran Dinesh K.,
Garcia Erwin,
Hua Jia,
Tabaczynski Walter,
Odunsi Kunle,
Andrews Chris,
Szyperski Thomas
Publication year - 2009
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.2469
Subject(s) - chemistry , analytical chemistry (journal) , spectrometer , protocol (science) , multivariate statistics , proton nmr , data collection , nuclear magnetic resonance spectroscopy , chromatography , statistics , organic chemistry , mathematics , medicine , physics , alternative medicine , quantum mechanics , pathology
Abstract A standard operating procedure (SOP) is presented for high‐throughput metabonomics studies using a 600 MHz NMR spectrometer equipped with a micro‐flow probe that is connected to an auto‐sampler. The procedure is designed to minimize random and systematic variation of NMR data collection. In addition, a protocol is described to assess the quality of the data acquired by a given NMR spectroscopist to ensure that (i) all researchers involved in the NMR data acquisition of a metabonomics research program perform equally and (ii) operator‐associated variation of NMR data collection is statistically not relevant for the interpretation of results obtained from multivariate data analyses. Copyright © 2009 John Wiley & Sons, Ltd.