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Spectral assignments and anisotropy data of cellulose I α : 13 C‐NMR chemical shift data of cellulose I α determined by INADEQUATE and RAI techniques applied to uniformly 13 C‐labeled bacterial celluloses of different Gluconacetobacter xylinus strains
Author(s) -
HesseErtelt Stephanie,
Witter Raiker,
Ulrich Anne S.,
Kondo Tetsuo,
Heinze Thomas
Publication year - 2008
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.2311
Subject(s) - cellulose , chemistry , anisotropy , nuclear magnetic resonance spectroscopy , carbon 13 nmr , chemical shift , bacterial cellulose , nmr spectra database , analytical chemistry (journal) , pulse sequence , spectroscopy , chemical structure , crystallography , spectral line , nuclear magnetic resonance , stereochemistry , organic chemistry , physics , optics , quantum mechanics , astronomy
Abstract Solid‐state 13 C‐NMR spectroscopy was used to characterize native cellulose pellicles from two strains of Gluconacetobacter xylinus (ATCC 53582, ATCC 23769), which had been statically cultivated in Hestrin–Schramm (HS) medium containing fully 13 C‐labeled β‐ D ‐glucose‐U‐ 13 C 6 as the sole source of carbon. For both samples, the 13 C‐NMR chemical shifts were completely assigned for each 13 C‐labeled site of cellulose I α with the aid of 2D refocused INADEQUATE NMR. To determine the principal chemical shift tensor components, a pulse sequence based on the recoupling of anisotropy information (RAI) was applied at 10 kHz MAS. The detailed 13 C tensors of cellulose I α from different bacterial celluloses are thus available now for the first time, and these results have been compared with previously published data of nonenriched material and with theoretical predictions. Copyright © 2008 John Wiley & Sons, Ltd.