Characterisation of uniformly  13 C,  15 N labelled bacteriochlorophyll  a  and bacteriopheophytin  a  in solution and in solid state: complete assignment of the  13 C,  1 H and  15 N chemical shifts | Zendy

EgorovaZachernyuk Tatiana | Zendy; van Rossum Barth | Zendy; Erkelens Cees | Zendy; de Groot Huub | Zendy

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Characterisation of uniformly 13 C, 15 N labelled bacteriochlorophyll a and bacteriopheophytin a in solution and in solid state: complete assignment of the 13 C, 1 H and 15 N chemical shifts

Author(s) -

EgorovaZachernyuk Tatiana,

van Rossum Barth,

Erkelens Cees,

de Groot Huub

Publication year - 2008

Publication title -

magnetic resonance in chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.483

H-Index - 72

eISSN - 1097-458X

pISSN - 0749-1581

DOI - 10.1002/mrc.2295

Subject(s) - chemistry , bacteriochlorophyll , photosynthesis , pigment , nuclear magnetic resonance spectroscopy , carbon 13 nmr , rhodopseudomonas , solid state , rhodospirillaceae , rhodopseudomonas palustris , photosynthetic reaction centre , photosynthetic pigment , stereochemistry , bacteria , organic chemistry , biochemistry , biology , genetics

In this investigation we report a complete assignment of 13 C, 1 H and 15 N solution and solid state chemical shifts of two bacterial photosynthetic pigments, bacteriochlorophyll (BChl) a and bacteriopheophytin (BPheo) a . Uniform stable‐isotope labelling strategies were developed and applied to biosynthetic preparation of photosynthetic pigments, namely uniformly 13 C, 15 N labelled BChl a and BPheo a . Uniform stable‐isotope labelling with 13 C, 15 N allowed performing the assignment of the 13 C, 15 N and 1 H resonances. The photosynthetic pigments were isolated from the biomass of photosynthetic bacteria Rhodopseudomonas palustris 17001 grown in uniformly 13 C (99%) and 15 N (98%) enriched medium. Both pigments were characterised by NMR in solution (acetone‐ d 6 ) and by MAS NMR in solid state and their NMR resonances were recorded and assigned through standard liquid 2D 13 C 13 C COSY, 1 H 13 C HMQC, 1 H 15 N HMBC and solid 2D 13 C 13 C RFDR, 1 H 13 C FSLG HETCOR and 1 H 15 N HETCOR correlation techniques at 600 MHz and 750 MHz. The characterisation of pigments is of interest from biochemical to pharmaceutical industries, photosynthesis and food research. Copyright © 2008 John Wiley & Sons, Ltd.

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