Premium
Characterization of nonderivatized plant cell walls using high‐resolution solution‐state NMR spectroscopy
Author(s) -
Yelle Daniel J.,
Ralph John,
Frihart Charles R.
Publication year - 2008
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.2201
Subject(s) - chemistry , kenaf , nuclear magnetic resonance spectroscopy , heteronuclear single quantum coherence spectroscopy , hibiscus , dissolution , cell wall , heteronuclear molecule , two dimensional nuclear magnetic resonance spectroscopy , characterization (materials science) , organic chemistry , botany , stereochemistry , nanotechnology , materials science , biology , fiber , biochemistry
A recently described plant cell wall dissolution system has been modified to use perdeuterated solvents to allow direct in‐NMR‐tube dissolution and high‐resolution solution‐state NMR of the whole cell wall without derivatization. Finely ground cell wall material dissolves in a solvent system containing dimethylsulfoxide‐ d 6 and 1‐methylimidazole‐ d 6 in a ratio of 4:1 (v/v), keeping wood component structures mainly intact in their near‐native state. Two‐dimensional NMR experiments, using gradient‐HSQC (heteronuclear single quantum coherence) 1‐bond 13 C 1 H correlation spectroscopy, on nonderivatized cell wall material from a representative gymnosperm pinus taeda (loblolly pine), an angiosperm Populus tremuloides (quaking aspen), and a herbaceous plant Hibiscus cannabinus (kenaf) demonstrate the efficacy of the system. We describe a method to synthesize 1‐methylimidazole‐ d 6 with a high degree of perdeuteration, thus allowing cell wall dissolution and NMR characterization of nonderivatized plant cell wall structures. Copyright © 2008 John Wiley & Sons, Ltd.