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Optimizing ssNMR experiments for dilute proteins in heterogeneous mixtures at high magnetic fields
Author(s) -
McNeill Seth A.,
Gor'kov Peter L.,
Struppe Jochem,
Brey William W.,
Long Joanna R.
Publication year - 2007
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.2146
Subject(s) - chemistry , nuclear magnetic resonance spectroscopy , selection (genetic algorithm) , solid state nuclear magnetic resonance , chemical physics , biological system , analytical chemistry (journal) , biochemical engineering , nuclear magnetic resonance , chromatography , organic chemistry , computer science , physics , artificial intelligence , biology , engineering
Solid‐state NMR spectroscopy at high magnetic fields is proving to be an effective technique in structural biology, particularly for proteins which are not amenable to traditional X‐ray and solution NMR approaches. Several parameters can be selected to provide optimal sensitivity, improve sample stability, and ensure biological relevance for ssNMR measurements on protein samples. These include selection of sample conditions, NMR probe design, and design of pulse experiments. Here, we demonstrate and evaluate several engineering and experimental approaches for pursuing measurements on dilute proteins in heterogeneous mixtures. Copyright © 2007 John Wiley & Sons, Ltd.