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Membrane orientation of the Na,K‐ATPase regulatory membrane protein CHIF determined by solid‐state NMR
Author(s) -
Franzin Carla M.,
Teriete Peter,
Marassi Francesca M.
Publication year - 2007
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.2144
Subject(s) - chemistry , solid state nuclear magnetic resonance , membrane , orientation (vector space) , solid state , atpase , crystallography , nuclear magnetic resonance , biochemistry , enzyme , geometry , mathematics , physics
Corticosteroid hormone‐induced factor (CHIF) is a major regulatory subunit of the Na,K‐ATPase, and a member of an evolutionarily conserved family of membrane proteins that regulate the function of the enzyme complex in a tissue‐specific and physiological‐state‐specific manner. Here we present the structure of CHIF oriented in the membrane, determined by solid‐state NMR orientation‐dependent restraints. Because CHIF adopts a similar structure in lipid micelles and bilayers, it is possible to assign the solid‐state NMR spectrum measured for 15 N ‐labeled CHIF in oriented bilayers from the structure determined in micelles, to obtain the global orientation of the protein in the membrane. Copyright © 2007 John Wiley & Sons, Ltd.
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