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1 H and 13 C NMR assignments for five anthraquinones from the mangrove endophytic fungus Halorosellinia sp. (No. 1403)
Author(s) -
Xia XueKui,
Huang HuaRong,
She ZhiGang,
Shao ChangLun,
Liu Fan,
Cai XiaoLing,
Vrijmoed L. L. P.,
Lin YongCheng
Publication year - 2007
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/mrc.2078
Subject(s) - anthraquinones , chemistry , mangrove , cytotoxicity , stereochemistry , fungus , two dimensional nuclear magnetic resonance spectroscopy , carbon 13 nmr , nmr spectra database , spectral line , botany , biochemistry , ecology , in vitro , biology , physics , astronomy
We report the unambiguous assignments of the 1 H and 13 C NMR spectra of two new natural products, namely, 1,4,5,6,7,9‐hexahydroxy‐2‐methoxy‐7‐methyl‐5β,9β,8aβ, 6α,10aα‐hexahydroanthracen‐10 (10aH)‐one (1) and 1,4,6‐trihydroxy‐2‐methoxy‐7‐methylanthracene‐9, 10‐dione (2), together with three known anthraquinones. These compounds were all isolated from the marine endophytic fungus No. 1403 collected from the South China Sea. Compounds 3 and 4 were isolated from the marine fungus for the first time. The structures were elucidated by the spectroscopic methods 1D and 2D NMR including COSY, HMQC, HMBC and NOE, and HREIMS. In our cytotoxicity assays, compound 5 showed cytotoxicity toward KB and KBv‐200 cells with IC 50 of 1.40 and 2.58 µg/ml, respectively. In addition, the plausible biogenic relationship of compounds 1, 2, 3 and 4 is discussed. Copyright © 2007 John Wiley & Sons, Ltd.